Identification, production, and purification of a novel lipase from Bacillus safensis
نویسندگان
چکیده
Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from Contaminated Soil a Palm Oil Processing ShedZainab Adenike Ayinla, Adedeji Nelson Ademakinwa, Femi Kayode Agboola
منابع مشابه
Lipase from Bacillus pumilus RK31: Production, Purification and Some Properties
A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange chromatograph...
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A gram-positive bacterium, denominated CFA-06, was isolated from Brazilian petroleum in the Campos Basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. The CFA-06 strain was identified as Bacillus safensis using the 16S rRNA and gyrase B sequence. Enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. T...
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Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...
متن کاملPurification and physicochemical properties of lipase from thermophilic Bacillus aerius.
A thermophilic bacterial isolate producing lipase was isolated from soil of hot spring and identified as Bacillus aerius (MTCC 10978). Peak lipase activity was observed when 30 h old inoculum was used and incubated in shaking conditions for 48 h. The optimal temperature and pH for the bacterial growth and lipase production was found to be 55°C and 8.0 respectively with cottonseed oil as carbon ...
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ژورنال
عنوان ژورنال: Journal of applied biology and biotechnology
سال: 2022
ISSN: ['2455-7005', '2347-212X']
DOI: https://doi.org/10.7324/jabb.2022.100410